IB Biology Substrate Specificity of Digestive Enzymes Planning Essay

IB Biology Substrate Specificity of Digestive Enzymes Planning investigation - Essay ExampleThere are two proposed mechanisms by which enzymes specificity can be put forward, 1) manoeuvre and key model where substrate act as key for enzyme as lock or in other words enzyme possess specific grooves where only specific substrate can be set(p) 2) Induced fix model where enzyme creates site for substrate when it comes in contact with it. In the given look into we will investigate the substrate specificity of enzyme amylase towards its substrate starch. We will also investigate the effect of various tangible parameters deal temperature, pH, enzyme concentration etc.3. Hypothesis Amylase has absolute specificity toward starch and will not act on any other polysaccharides. Similarly rate of conversion of starch to glucose is highly influenced by various factors like temperature, enzyme concentration and pH.The furnishs were arranged and 5ml of Starch, Cellulose and glycogen firmness o f purpose was added to them and incubated at 37C in water bath for 2-3min to allow the temperature of solution reach to 37C (water bath was switch on before experiment was started and was at set temperature).Tubes were ascertained for wild to brick red color indicating liberation of glucose. Appropriate blanks were kept to validate overall experimental procedure that includes Starch solution without amylase, tubes without starch or substrate etc. Development of red color indicates activity of Amylase and thus generation of glucose.7. Control variablesTemperature Test tubes were arranged as mentioned earlier and 5ml of starch solution was added to them. Three disparate temperatures were used in this experiment. The water baths were set at 20C, 37C and 45C. When the substrate attained the temperature, 1ml of saliva was added to each tube and further incubated for 15min. After incubation few drops of benedicts reagents were added to each and further incubated for 5 min. Color develo pment was observed as above and noted.